Abstract

Cucumber mosaic virus (CMV) subgroup IA isolates were first identified from Nandina domestica plants in Zhejiang province of China by small RNA deep sequencing followed by bioinformatics analysis and was confirmed by RT-PCR amplification, 5′ and 3′ RACE and DNA sequencing. The complete nucleotide sequences of this virus shared the highest sequence similarities (97–98%) with CMV subgroup IA isolate CTL RNA1 and RNA3 (GenBank accession no: EF213023, EF213025) and RP48 RNA2 (KC527727), respectively; and converged with CMV subgroup IA isolates into a branch in phylogenetic tree using full length nucleotide sequences. Pathogenicity test confirmed that this virus can be mechanically transmitted to N. domestica, Nicotiana tabacum and N. benthamiana. All thirty randomly collected samples of four major N. domestica varieties (N. domestica cv. Hong Baoshi, Nana, Fire Power and Xiu Dada) in Lin'an Zhejiang province were positive for CMV subgroup IA. This is the first report of CMV subgroup IA isolate on varieties of N. domestica in China.