Efficient regio- and stereo-selective C–H bond hydroxylation of steroids using an engineered heme-thiolate peroxygenase biocatalyst

Catal. Sci. Technol., 2023, Advance Article
DOI: 10.1039/D3CY01223A, Communication
Jinia Akter, Eva F. Hayball, Stephen G. Bell
A steroid hydroxylating monooxygenase enzyme was converted into a peroxygenase to enable the stereoselective hydroxylation of C–H bonds in these complex molecules simply by adding hydrogen peroxide.
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