Accurate quantification of DNA content in DNA hydrogels prepared by rolling circle amplification

Chem. Commun., 2023, 59,12184-12187
DOI: 10.1039/D3CC04374F, Communication
Open Access Open Access
Creative Commons Licence  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Leonie Schneider, Madleen Richter, Claude Oelschlaeger, Kersten S. Rabe, Carmen M. Domínguez, Christof M. Niemeyer
Accurate quantification of amplified DNA in RCA-based hydrogels is achieved by a photometric nucleotide depletion assay or qPCR. The DNA content strongly depends on the template sequence and correlates with the mechanical properties of the hydrogels.
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