Development of high methanol-tolerance Pichia pastoris based on iterative adaptive laboratory evolution

Green Chem., 2023, Advance Article
DOI: 10.1039/D3GC02874G, Paper
Shuai Wang, Yuanyuan Wang, Qingyan Yuan, Liu Yang, Fengguang Zhao, Ying Lin, Shuangyan Han
High methanol-tolerant strains were obtained using iterative adaptive laboratory evolution (microbial microdroplet culture (MMC) and shake flask culture (SFC)).
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