Detecting secondary structure formation with FRET-PAINT

Analyst, 2023, 148,4655-4658
DOI: 10.1039/D3AN01118F, Communication
Sineth G. Kodikara, Kylie J. Merkel, Simon J. Haas, Sajad Shiekh, Hamza Balci
Accessibility of a nucleic acid to a complementary imager strand (Cy5-PNA) decreases when it forms a secondary structure (G-Triplex). This provides a way to detects structure formation regardless of how small the change in end-to end separation is.
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