Establishing stereochemical comparability in phosphorothioate oligonucleotides with nuclease P1 digestion coupled with LCMS analysis

Analyst, 2023, Advance Article
DOI: 10.1039/D3AN01392H, Communication
Zifan Li, Fei Tong, Li Xiao, Nicholas R. Larson, Xuan Zhou, Yueheng Zhang, Jonas P. Immel-Brown, George M. Bou-Assaf
NP1 digestion coupled with UHPLC-HRMS can provide valuable information on stereochemical comparability between phosphorothioate oligonucleotides by examining digestion fragments.
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