A feasible protocol to profile bile acids in dried blood spots from rats using a UHPLC-MS/MS method combining a surrogate matrix

Analyst, 2023, 148,5190-5202
DOI: 10.1039/D3AN00900A, Paper
Ziying Ma, Ning Sheng, Jinlan Zhang
A two-step method was applied to enable high extraction efficiency for bile acids in DBSs. A DBS surrogate matrix was well developed and showed good performance, and 35 bile acids were profiled using UHPLC-MS/MS with good resolution and quantified with acceptable precision and accuracy.
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