Label-free analysis of membrane protein binding kinetics and cell adhesions using evanescent scattering microscopy

Analyst, 2023, 148,5084-5093
DOI: 10.1039/D3AN00977G, Paper
Jiying Xu, Caixin Huang, Liangju Li, Ying Zhao, Zhenpeng Guo, Yi Chen, Pengfei Zhang
A surface-sensitive imaging approach is developed for imaging the cell adhesion sites with high resolution and membrane protein binding kinetics in single live cells.
The content of this RSS Feed (c) The Royal Society of Chemistry

Aptamer-functionalized two-photon SiO2@GQDs hybrid-based signal amplification strategy for targeted cancer imaging

Analyst, 2023, 148,5124-5132
DOI: 10.1039/D3AN01393F, Paper
Huijuan Yan, Shuo Yang, Mengxue Liu, Ke Bao, Wu Ren, Fei Lin, Yiqiao Gao, Zhenghui Wang, Shuanghui Liu, Jieli Lv, Ying Zhao
A versatile strategy has been employed to fabricate an aptamer Sgc8c-functionalized hybrid as a proof-of-concept of the signal amplification strategy for targeted cancer imaging.
The content of this RSS Feed (c) The Royal Society of Chemistry

Ratiometric fluorescent aptasensor for convenient detection of ochratoxin A in beer and orange juice

Analyst, 2023, 148,5172-5177
DOI: 10.1039/D3AN01360J, Paper
Jie Yu, Shuheng Ai, Wenhan Zhang, Chao Wang, Pengfei Shi
Based on the principle of fluorescence resonance energy transfer (FRET), a simple ratiometric fluorescent aptasensor for convenient detection of ochratoxin A (OTA), a group IIB carcinogen secreted by some fungi, was established.
The content of this RSS Feed (c) The Royal Society of Chemistry

Characterization of site-specific N-glycosylation signatures of isolated uromodulin from human urine

Analyst, 2023, 148,5041-5049
DOI: 10.1039/D3AN01018J, Paper
Tianhai Lin, Zhuo Chen, Mengqi Luo, Yang Zhao, Wenjuan Zeng, Shanshan Zheng, Tao Su, Yi Zhong, Shisheng Wang, Youmei Jin, Liqiang Hu, Wanjun Zhao, Jiaxu Li, Xuanyi Wang, Changwei Wu, Dapeng Li, Fang Liu, Guisen Li, Hao Yang, Yong Zhang
Uromodulin (Umod, Tamm–Horsfall protein) is the most abundant urinary N-glycoprotein produced exclusively by the kidney.
The content of this RSS Feed (c) The Royal Society of Chemistry

A lysosome-targeted triazole near-infrared cyanine fluorescent probe for in vivo long-term cell tracking

Analyst, 2023, 148,5117-5123
DOI: 10.1039/D3AN01238G, Paper
Xianrong Yu, Yu Wu, Wei Tang, Xinrui Duan
A lysosome-targeted triazole NIR cyanine fluorescent probe IR780-NT-NH2 with high photostability and brightness, excellent lysosomal targeting ability, and ideal cellular retention ability was developed for in vivo long-term cell tracking.
The content of this RSS Feed (c) The Royal Society of Chemistry

Exo I signal amplification of a DNA hydrogel film combined with capillary self-driven action for EpCAM detection

Analyst, 2023, 148,4730-4737
DOI: 10.1039/D3AN01011B, Paper
Shuang Li, Zhiguang Wang, Xiaoxiao Lin, Yalan Bian, Liqun Chen
A DNA hydrogel film is combined with capillary self-driven action for the specific detection of the tumor marker EpCAM, and Exo I is further introduced for signal amplification.
The content of this RSS Feed (c) The Royal Society of Chemistry

Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Analyst, 2023, 148,4939-4953
DOI: 10.1039/D3AN01201H, Critical Review
Yidan Mo, Huangmei Zhou, Jinming Xu, Xihang Chen, Lei Li, Sanjun Zhang
This review outlines the overview, advances and opportunities of genetically encoded fluorescence lifetime biosensors, including their combination of lifetime imaging technique and lifetime analysis methods, applications and future consideration.
The content of this RSS Feed (c) The Royal Society of Chemistry

Broadband ultrafast 2D NMR spectroscopy for online monitoring in continuous flow

Analyst, 2023, 148,5255-5261
DOI: 10.1039/D3AN01165H, Paper
Célia Lhoste, Margherita Bazzoni, Justine Bonnet, Aurélie Bernard, François-Xavier Felpin, Patrick Giraudeau, Jean-Nicolas Dumez
2D NMR spectra with full spectra width are obtained from continuously flowing samples in less than 90 s, using interleaved ultrafast NMR acquisition.
The content of this RSS Feed (c) The Royal Society of Chemistry

Exceptional peroxidase-like activity of an iron and copper based organic framework nanosheet for consecutive colorimetric biosensing of glucose and kanamycin in real food samples

Analyst, 2023, 148,5157-5171
DOI: 10.1039/D3AN01242E, Paper
Rajakumari Jesuraj, Arunjegan Amalraj, Vinoth Kumar Vaidyanathan, Panneerselvam Perumal
Two-dimensional metal–organic framework nanosheets are attractive as peroxidase mimicking nanocatalysts due to their rich chemical functional groups, large surface area, high porosity, and accessible active sites.
The content of this RSS Feed (c) The Royal Society of Chemistry