Two novel Al3+ fluorescent-on probes based on thiazole substituted salicylaldehyde and its applications

Posted on 23 October 2023 by

Volume 34, Issue 3-4, March - April 2023, Page 158-167
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Factors Affecting the Extraction of (Poly)Phenols from Natural Resources Using Deep Eutectic Solvents Combined with Ultrasound-Assisted Extraction

Posted on 18 October 2023 by

Unlocking the Hidden Depths: Multi-Modal Integration of Imaging Mass Spectrometry-Based and Molecular Imaging Techniques

Posted on 17 October 2023 by

Quasicrystal Structure Prediction: A Review

Posted on 16 October 2023 by nMichael Widom, nMarek Mihalkovičn

Abstract

Predicting quasicrystal structures is a multifaceted problem that can involve predicting a previously unknown phase, predicting the structure of an experimentally observed phase, or predicting the thermodynamic stability of a given structure. We survey the history and current state of these prediction efforts with a focus on methods that have improved our understanding of the structure and stability of known metallic quasicrystal phases. Advances in the structural modeling of quasicrystals, along with first principles total energy calculation and statistical mechanical methods that enable the calculation of quasicrystal thermodynamic stability, are illustrated by means of cited examples of recent work.

A Quantitative Sequencing Method for 5‐Formylcytosine in RNA

Posted on 16 October 2023 by

Abstract

5-Formylcytosine (f⁵C) modification is present in human mitochondrial methionine tRNA (mt-tRNA^Met) and cytosolic leucine tRNA (ct-tRNA^Leu), with their formation mediated by NSUN3 and ALKBH1. f⁵C has also been detected in yeast mRNA and human tRNA, but its transcriptome-wide distribution in mammals has not been studied. Here we report f⁵C-seq, a quantitative sequencing method to map f⁵C transcriptome-wide in HeLa and mouse embryonic stem cells (mESCs). We show that f⁵C in RNA can be reduced to dihydrouracil (DHU) by pic-borane, and DHU can be exclusively read as T during reverse transcription (RT) reaction, allowing the detection and quantification of f⁵C sites by a unique C-to-T mutation signature. We validated f⁵C-seq by identifying and quantifying the two known f⁵C sites in tRNA, in which the f⁵C modification fractions dropped significantly in ALKBH1-depleted cells. By applying f⁵C-seq to chromatin-associated RNA (caRNA), we identified several highly modified f⁵C sites in HeLa and mouse embryonic stem cells (mESC).