Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Analyst, 2023, 148,4939-4953
DOI: 10.1039/D3AN01201H, Critical Review
Yidan Mo, Huangmei Zhou, Jinming Xu, Xihang Chen, Lei Li, Sanjun Zhang
This review outlines the overview, advances and opportunities of genetically encoded fluorescence lifetime biosensors, including their combination of lifetime imaging technique and lifetime analysis methods, applications and future consideration.
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Exo I signal amplification of a DNA hydrogel film combined with capillary self-driven action for EpCAM detection

Analyst, 2023, 148,4730-4737
DOI: 10.1039/D3AN01011B, Paper
Shuang Li, Zhiguang Wang, Xiaoxiao Lin, Yalan Bian, Liqun Chen
A DNA hydrogel film is combined with capillary self-driven action for the specific detection of the tumor marker EpCAM, and Exo I is further introduced for signal amplification.
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A lysosome-targeted triazole near-infrared cyanine fluorescent probe for in vivo long-term cell tracking

Analyst, 2023, 148,5117-5123
DOI: 10.1039/D3AN01238G, Paper
Xianrong Yu, Yu Wu, Wei Tang, Xinrui Duan
A lysosome-targeted triazole NIR cyanine fluorescent probe IR780-NT-NH2 with high photostability and brightness, excellent lysosomal targeting ability, and ideal cellular retention ability was developed for in vivo long-term cell tracking.
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Characterization of site-specific N-glycosylation signatures of isolated uromodulin from human urine

Analyst, 2023, 148,5041-5049
DOI: 10.1039/D3AN01018J, Paper
Tianhai Lin, Zhuo Chen, Mengqi Luo, Yang Zhao, Wenjuan Zeng, Shanshan Zheng, Tao Su, Yi Zhong, Shisheng Wang, Youmei Jin, Liqiang Hu, Wanjun Zhao, Jiaxu Li, Xuanyi Wang, Changwei Wu, Dapeng Li, Fang Liu, Guisen Li, Hao Yang, Yong Zhang
Uromodulin (Umod, Tamm–Horsfall protein) is the most abundant urinary N-glycoprotein produced exclusively by the kidney.
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Ratiometric fluorescent aptasensor for convenient detection of ochratoxin A in beer and orange juice

Analyst, 2023, 148,5172-5177
DOI: 10.1039/D3AN01360J, Paper
Jie Yu, Shuheng Ai, Wenhan Zhang, Chao Wang, Pengfei Shi
Based on the principle of fluorescence resonance energy transfer (FRET), a simple ratiometric fluorescent aptasensor for convenient detection of ochratoxin A (OTA), a group IIB carcinogen secreted by some fungi, was established.
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Aptamer-functionalized two-photon SiO2@GQDs hybrid-based signal amplification strategy for targeted cancer imaging

Analyst, 2023, 148,5124-5132
DOI: 10.1039/D3AN01393F, Paper
Huijuan Yan, Shuo Yang, Mengxue Liu, Ke Bao, Wu Ren, Fei Lin, Yiqiao Gao, Zhenghui Wang, Shuanghui Liu, Jieli Lv, Ying Zhao
A versatile strategy has been employed to fabricate an aptamer Sgc8c-functionalized hybrid as a proof-of-concept of the signal amplification strategy for targeted cancer imaging.
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Label-free analysis of membrane protein binding kinetics and cell adhesions using evanescent scattering microscopy

Analyst, 2023, 148,5084-5093
DOI: 10.1039/D3AN00977G, Paper
Jiying Xu, Caixin Huang, Liangju Li, Ying Zhao, Zhenpeng Guo, Yi Chen, Pengfei Zhang
A surface-sensitive imaging approach is developed for imaging the cell adhesion sites with high resolution and membrane protein binding kinetics in single live cells.
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A smart chitosan-graphite molecular imprinted composite for the effective trapping and sensing of dimethyl methylphosphonate based on changes in resistance

Analyst, 2023, 148,5012-5021
DOI: 10.1039/D3AN01293J, Paper
Open Access Open Access
James Disley, Guzmán Gil-Ramírez, Peter Eaton, Jose Gonzalez-Rodriguez
A chitosan-graphite molecular imprinted polymer able to trap and detect dimethyl methylphosphonate (DMMP) through a change in resistance has been produced.
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Detecting secondary structure formation with FRET-PAINT

Analyst, 2023, 148,4655-4658
DOI: 10.1039/D3AN01118F, Communication
Sineth G. Kodikara, Kylie J. Merkel, Simon J. Haas, Sajad Shiekh, Hamza Balci
Accessibility of a nucleic acid to a complementary imager strand (Cy5-PNA) decreases when it forms a secondary structure (G-Triplex). This provides a way to detects structure formation regardless of how small the change in end-to end separation is.
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