Category Archives: Animal Science Journal

Effects of different coating materials on the morphological characteristics of chicken adenohypophyseal folliculo‐stellate cells in vitro

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Abstract

Chicken adenohypophyseal cells were cultured in plates coated with different materials, and their morphologies were examined to confirm the characteristics of chicken folliculo-stellate (FS) cells in vitro. The adenohypophyseal cells were dispersed with a collagenase/trypsin mixture in media and seeded in plates coated in either poly L-lysine (PLL), collagen, or laminin. After 7 days of culture, the cells were fixed and immunocytochemistry was performed. 5-Bromo-2′-deoxyuridine incorporation test indicated that the proliferation activity of the culture cells was different based on the coating materials, and it was higher in the collagen-coated plate than two other coating materials. Fluorescence immunocytochemistry was also performed using mixed antibodies against growth hormone, prolactin, luteinizing hormone β-subunit, basic cytokeratin (bCK), and S100B. The culture cells on the PLL- and laminin-coated surfaces were round or oval in shape, and bCK-immunopositive FS cells were morphologically indistinguishable from endocrine cells. In the collagen-coated plate, many endocrine cells were round or oval in shape, but FS cells displayed a larger and flattened morphology. S100B-immunoreactions were localized in the nuclei of bCK-immunopositive FS cells. These results suggest that culturing the chicken adenohypophyseal cells in the collagen-coated plate enables the distinction of FS cells from endocrine cells.

Differences in ingestive behavior between grazing goats and sheep at the bite scale

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Abstract

This study aimed to clarify the differences in ingestive behavior between goats and sheep at the bite scale. Animals were allowed to graze a feeding station of bahiagrass (Paspalum notatum Flügge) with two sward heights (short and tall) to two levels of bite depletion (20 and 40 bites). Ingestive behavior variables, which represent the rate and mass of bites and chews, intake rate, bite dimensions, and bite placement characteristics, were quantified. The interspecies difference in ingestive behavior became obvious at the short sward. Goats reduced the inter-bite distance and maintained bite overlapping but took deeper bites than sheep at the short sward, resulting in slightly greater bite mass. These behavioral changes with efficient chewing may be helpful to maintain the intake rate at the short sward. Sheep maintained their inter-bite distance regardless of the sward height and decreased overlapping bites at the short sward. These behavioral characteristics in sheep in response to sward height may partly compensate for the decrease in the bite mass at the short sward, although it may not always maintain the intake rate. Thus, the present study suggests that goats and sheep implement different bite scale ingestive behaviors, including bite placement, when they eat short swards.

Diversity of mitochondrial D‐loop haplotypes from ancient Thracian horses in Bulgaria

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Abstract

The domestication of the horse began possibly more than 5000 years ago in the western part of the Eurasian steppe, and according to the leading hypothesis, horses first spread from the Steppe toward the region of the Thracian culture, starting in the second half of the 2nd millennium BCE and flourished from the fifth to first centuries BCE, mainly located in present-day Bulgaria. We analyzed 17 horse bone remains excavated from Thracian archaeological sites (fourth to first centuries BCE) in Bulgaria and successfully identified 17 sequences representing 14 different haplotypes of the mitochondrial D-loop. Compared with the mtDNA haplotypes of modern horses around the world, ancient Thracian horses in Bulgaria are thought to be more closely related to modern horses of Southern Europe and less related to those of Central Asia. In addition, the haplotypes we obtained represented 11 previously reported modern horse mtDNA haplogroups: A, B, D, E, G, H, I, L, N, P, and Q. All the haplogroups contain modern and regionally predominant haplotypes occurring in Europe, the Middle East, and Central Asia. Our results indicate that Thracian horses in Bulgaria have had relatively high genetic diversity and are closely related to modern horse breeds.

Interactive effects of dietary supplementation between illite and probiotic on productive performance, intestinal microflora, and blood profiles of laying hens

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Abstract

This study investigates the interactive effects of dietary illite and probiotic on productive performance, intestinal microflora, and blood profiles of laying hens. A total of 432 laying hens at 24 weeks old were allotted into six dietary groups. An experimental design was composed with a 3 (illite levels: 0, 0.3, and 0.6%) × 2 (probiotic levels: 0 and 0.2%). The probiotic based on Bacillus subtilis, Saccharomyces cerevisiae, and B. licheniformis was used. There were interactions between illite and probiotic on total microbes and salmonella of digesta. Higher egg production was observed in hens fed a diet supplemented with either 0.6% illite or 0.2% probiotic than in those fed a basal diet. The total microbes of the group fed a diet with 0.6% illite were lower than the groups fed diets with 0 and 0.3% illite. A lower number of Escherichia coli was observed in hens fed a diet with probiotic than those fed a basal diet. Higher immunoglobulin G concentration was observed in the group fed a diet supplemented with 0.6% illite than in those fed a basal diet. Our results suggest illite and probiotic can be used as feed additives for hens, separately or in combination to improve performance and intestinal microflora.

Bayesian analysis of genetic and environmental effects on litter traits in a red fox (Vulpes vulpes) herd under long‐term selection

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Abstract

The economic efficiency of fur animal farms is considerably influenced by reproductive performance. The objectives of this study are to determine the effects of individual and maternal inbreeding, birth year, and dam and sire age on litter size at birth (LSB) and at weaning (LSW) and on preweaning mortality (PWM) in a red fox herd under long-term selection, and to determine the heritability of these traits. In total, 37,973 pedigreed individuals were used to calculate the inbreeding coefficients, based on records of 14,527 litters of 3856 dams born from the year 1958 to 2015. Two data sets (all data and data for the Polish variety) were analyzed. The highest heritability was estimated for PWM (0.292, 0.306) and the lowest for LSW (0.114, 0.115). In contrast to paternal and maternal inbreeding, litter inbreeding was found to exert a significant influence. The absence of significant effects of most varieties may suggest relatively large genetic similarity in the world red fox population. This corresponds with the similarity of the results obtained for the total herd and for the Polish variety. Favorable genetic trends were observed for the studied traits, indicating that the selection applied had been a relatively effective approach to improving these traits.

Estimation of genetic parameters for production, reproduction, and growth curve of Fars indigenous chicken

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Abstract

Iranian native chicken, including Fars indigenous chicken, is an important genetic resource due to its adaptation to stressful environmental conditions, good endurance and resistance to disease. The aim of this research was to determine the genetic infrastructure of Fars indigenous chicken using several nonlinear functions. The dataset included body weight at hatch (BW1), body weight at the 8th week (BW8), body weight at the 12th week (BW12), weight at sexual maturity (WSM), age at sexual maturity (ASM), number of eggs in the first 12 weeks of laying period (EN), egg weight at the first day of laying (EW1), average egg weight at the 28thday of laying (EW28), and average egg weight at weeks 28, 30, and 32 of the laying period (AEW). Growth models were fitted using the NLIN procedure and WOMBAT software was used to predict variance components for the best fit model parameters. Results suggested three-parameter models, for example, Gompertz, fitted better to the data than others. The maturity weight (A), initial weight (B), and maturity rate (K) parameters in the Gompertz model were 1996.8 ± 6.63, 4.11 ± 0.03, and 0.021 ± 0.0001, respectively. The heritability of A, B, and K parameters were 0.03, 0.05, and 0.12, respectively.

Effect of different odors on the fighting behavior of weaning piglets after merging into a large pen

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Abstract

In intensive pig production, the fighting behavior of weaning piglets after merging pens is relatively common. Fighting behavior not only easily causes injury in pigs but also affects the production performance of pigs. To reduce fighting behavior in farms, this study aimed to explore the possible effect of odorous substances on piglet fighting behavior after merging into a large pen. Six different sprays were tested: original creamy, cheese flavor, orange flavor, truffle, vanilla and pigpen flavor. In each experiment, two groups were set (one odor-sprayed and no sprayed control), and 12 pigs were used per group. After mixing, the frequency of occurrence of various piglet behaviors in different pens was recorded. During this period, salivary cortisol levels and skin lesion scores were evaluated. As a result, the piglets sprayed with the original creamy, cheese flavor and vanilla substances obtained significantly higher average daily gain and feed intake and showed a significantly lower incidence of fighting behavior, and the skin lesion score and salivary cortisol of piglets were also reduced significantly. All the other odorous substances had no significant effects on the fighting behavior and production performance of piglets.

Reduction of oocyte shedding and cecal inflammation by 5‐aminolevulinic acid daily supplementation in laying hens infected with Eimeria tenella

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The present study aimed to evaluate the effects of 5-aminolevulinic acid (5-ALA) on Eimeria tenella infection in laying hens. Oocyst shedding and histopathology were evaluated. A reduced oocyst shedding was observed 5 and 7 days post-infection (dpi) in the 5-ALA-administered group, but the total number of oocysts during the first infection period was not different between control and 5-ALA-treated groups. After E. tenella attack infection, the period of oocyst shedding in the 5-ALA-administered group lasted less long than that in controls. During the attack infection period, the total number of fecal oocysts in the 5-ALA-treated group was significantly lower than that in the control group. However, the parasite burden score in hens receiving 5-ALA was higher than that in controls after E. tenella attack infection. The lesion scores at 5 and 30 dpi in the control group were significantly lower than those in the 5-ALA-administered group. Therefore, 5-ALA administration might be beneficial against E. tenella infection in laying hens.

Effect of the gain rate during growing phase on the performance and characteristics of carcass and meat of super precocious steers

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The aim was to evaluate the effect of different rates of weight gain during the backgrounding on animal performance and carcass and meat characteristics of steers finished in feedlots. Thirty-six Angus steers, 12 ± 2 months of age, were backgrounded during 91 days on Aruana grass pasture (Panicum maximum cv. Aruana) managed under different stocking rates to achieve three different weight gains: HIGH ADG (average daily gain)—no feed restriction (ADG = 0.846 kg); MEDIUM ADG—moderate feed restriction (ADG = 0.456 kg); and LOW ADG—high feed restriction (ADG = 0.154 kg). To ensure the difference in ADG, we offered 0.7% live weight of concentrate feed in the HIGH treatment and a better forage supply in the MEDIUM treatment. After the backgrounding, the animals were finished in feedlot. There was no effect of the previous performance on the animals' performance in the feedlot. The LOW presented higher weight (218.9 vs. 207.9 kg) and hot (54.0% vs. 51.3%) and cold (53.5% vs. 50.5%) carcass yield than the MEDIUM, besides presenting meat with less cooking losses (15.0% vs. 18.2%), marbling (7 vs. 4.0 points), palatability (7.1 vs. 6.3 points), juiciness (7.2 vs. 6.4 points), tenderness (7.1 vs. 6.3 points), and lower shear force (5.78 vs. 8.75 kg) than HIGH. LOW ADG steers stay longer in the finishing phase but presented in general better quality carcass and meat than those with MEDIUM or HIGH during the backgrounding.